Navegando por Autor "Bermejo, Gabriela Rastelli"

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    Diversidade genética de isolados brasileiros de Phakopsora pachyrhizi (Sydow & Sydow)
    (Universidade Estadual do Norte do Paraná, 2016-03-30) Bermejo, Gabriela Rastelli; Carvalho, Mayra Costa da Cruz Gallo de; https://orcid.org/0000-0002-1559-3828; http://lattes.cnpq.br/2844751503530944; Carvalho, Mayra Costa da Cruz Gallo de; https://orcid.org/0000-0002-1559-3828; http://lattes.cnpq.br/2844751503530944; Carvalho, Sandremir de; https://orcid.org/0000-0002-4830-5722; http://lattes.cnpq.br/8684697240694261; Podanosqui, Adriana Maria Polizel; http://lattes.cnpq.br/2507513800168563; Matsumoto, Leopoldo Sussumu; https://orcid.org/0000-0001-5102-545X; http://lattes.cnpq.br/0857955043436449; Marcelino-Guimarães, Francismar Corrêa; https://orcid.org/0000-0002-5828-7249; http://lattes.cnpq.br/4585015105548818
    Asian soybean rust (ASR) caused by obligate biotrophic fungus Phakopsora pachyrhizi Syd. & P. Syd., Is a major disease that affects the culture of soybean [Glycine max (L.) Merr.], Causing losses in production in many producing areas of the world. The specific resistance to P. pachyrhizi fungus was found, so far, in six locus, called Rpp1, Rpp1b, Rpp2, Rpp3, Rpp4, Rpp5 and Rpp6. However this locus does not confer full resistance to the pathogen, hindering the development of effective cultivars. Therefore, knowledge of the genetic variability in populations of P. pachyrhizi and isolates are essential for breeding programs can perform an efficient strategic planning against the fungus that causes Asian soybean rust. Therefore, the objective of this study was to extend analysis initiated by Darben et al. (2012), from the use of dominant and co dominant markers to estimate the molecular diversity between monourediniais isolates of P. pachyrhizi and to a population of spores of the fungus. After successive cycles monouredinial culture in a separate leaves were obtained DNA six isolated monourediniais and one population collected in soybean producing regions in the country. The amplification seven pairs of AFLP primers showed an average genetic diversity was between pairs from 21.88% -77.98% (NIS), the greater diversity in pairs found between the isolated LLRV212 (MT) and the population (PR). The highest similarity was found between 80.35% LCA4A12 isolated (MG) and LLRV212 (MT). The Isolated LGO 112 (GO) presented 30 unique brands and not clumped together in the cluster formed by the other. In terms of inoculum selection, molecular results indicated this material as a major material to be considered for virulence phenotype analysis and aggressiveness. The five amplified SSR primers showed no polymorphism. It is possible to suggest that at least five of the seven tested materials represent sources of genetically distinct inoculations. It will now be essential to observe the molecular variation corresponds to some degree the phenotypic variation observed in soybean plants differentiated response on the different materials of the fungus.