Navegando por Autor "Macedo, Mariana Ferracin de"

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    Identificação molecular e caracterização morfológica do isolado de nematoide entomopatogênico Heterorhabditis sp. Uenpn-01 obtido em área de pastagem no município de Ribeirão Claro, Paraná
    (Universidade Estadual do Norte do Paraná, 2020) Macedo, Mariana Ferracin de; Alves, Viviane Sandra; https://orcid.org/0000-0003-2381-8115; http://lattes.cnpq.br/3794580975938452; Alves, Viviane Sandra; https://orcid.org/0000-0003-2381-8115; http://lattes.cnpq.br/3794580975938452; Guide, Bruna Aparecida; http://lattes.cnpq.br/7288306329944786; Ferreira, Dhiego Gomes; https://orcid.org/0000-0003-4375-6556; http://lattes.cnpq.br/7326714947358202
    Nematodes are worms belonging to the Phylum Nematoda, which have diversified habits, with parasitic groups of plants, human parasites and also insect parasitic nematodes, known as entomopathogenic nematodes (NEPs). NEPs can be found practically all over the world, being isolated from different ecosystems from soil samples, or from infected insects found in the field, and in Brazil, there are approximately 54 registered isolates. In the state of Paraná, there is only one species of entomopathogenic nematode, Heterorhabditis amazonensis, showing the importance and the need to better understand this group of entomopathogens in our state. Thus, the objective of this work was to identify and characterize a nematode isolate belonging to the genus Heterorhabditis sp., Isolated in the city of Ribeirão Claro, PR. The isolation was obtained by collecting soil, and the technique of "live baits" with caterpillars of Galleria mellonella (Lepidoptera: Pyralidae) was used. The molecular identification of the NEP isolate was carried out by amplifying the ITS region and also the D2D3 region, as well as by measuring structures for morphological identification. Scanning Electron Microscopy (SEM) photos were also taken to analyze morphological structures. In addition, the pathogenicity of G. mellonella caterpillars and observation of the life cycle were evaluated. In the molecular identification process from the ITS region, 98.64% similarity was observed with Heterorhabditis mexicana and 98.57% with Heterorhabditis taysearae, and in the D2D3 region, 96.94% similarity with H. mexicana. Regarding the morphological identification, it was observed that the isolate presents differences in measurements when compared to H. mexicana, however it has a similar characteristic in males in which the excretory pore is located posteriorly to the basal bulb. In the SEM photos, it is possible to observe structures of the hermaphroditic females, second generation females and males, observing the presence of the labial papillae in all NEPs in the anterior region. In females, the vulva is observed in the median region of the body, as well as the tail and anus in the posterior region. In males, in the posterior part, the tail and spike can be noticed. In the pathogenicity test, the isolate caused 88% mortality of G. mellonella caterpillars, and as for the life cycle, the UENPn-01 isolate presented a cycle characteristic of the genus Heterorhabditis, with the first generation of hermaphrodite females only, followed by three generations with the presence of males and females, totaling four complete generations within the host.