Navegando por Autor "Moreira, Giovanna Melatti Bermal"

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    Avaliação do extrato hidroalcoólico de Punica granatum linn. sobre bactérias de interesse à mastite bovina
    (Universidade Estadual do Norte do Paraná, 2014-08-07) Moreira, Giovanna Melatti Bermal; Matsumoto, Leopoldo Sussumu; https://orcid.org/0000-0001-5102-545X; http://lattes.cnpq.br/0857955043436449; Peixoto, Erika Cosendey Toledo de Mello; https://orcid.org/0000-0002-9608-4282; http://lattes.cnpq.br/0963818563395019; Peixoto, Erika Cosendey Toledo de Mello; https://orcid.org/0000-0002-9608-4282; http://lattes.cnpq.br/0963818563395019; Silva, Luciana Pereira; https://orcid.org/0000-0002-0914-2094; http://lattes.cnpq.br/8207280726883591; Silva, Regildo Márcio Gonçalves da; http://lattes.cnpq.br/5285324361162578; Patelli, Thais Helena Constantino; https://orcid.org/0000-0002-2981-6471; http://lattes.cnpq.br/6738089449088570; Silva, Marcelo Alves da; http://lattes.cnpq.br/3283906522734749
    Bovine mastitis is characterized by inflammation of the mammary gland, usually due to bacterial infection, compromising quantity and quality of milk production. This study aimed to determine the antibacterial activity in vitro of the hydroalcoholic extract of the bark of the pomegranate fruit on standard strains of interest the bovine mastitis. The colonies were resuspended and adjusted at a concentration of 107 ml-1 UV-visible spectrophotometry. The extracts were evaluated in five replications in seven concentrations: 1000 mg mL-1to 25 mg mL-1. Were used strains of Staphylococcus aureus ATCC 25923, Staphylococcus saprophyticus ATCC 15305, Staphylococcus epidermidis ATCC 12228, Escherichia coli ATCC 11229, Pseudomonas aeruginosa ATCC 27853, Salmonella enterica subsp. serovar Typhi ATCC 19214, Enterobacter cloacae ATCC 23355 and Bacillus cereus ATCC 33018. Sensitivity of the strains were determined using the minimum inhibitory concentration and disk diffusion test and the results showed that the inhibition zones corresponding to values from 15 mm were considered sensitive. For phytochemical evaluation was determined levels of antioxidant activity, total phenols and flavonoids. Thus, the extract was diluted in seven concentrations: 25 the 1000 mg L-1, and evaluated in triplicate. Bacterial growth was inhibited at concentrations of 100 mg mL-1 and the antioxidant activity was observed from 50 mg mL-1, with values corresponding to 4,62% to 64,90% plateau at a concentration of 500 mg mL-1. In the evaluation of radical scavenging activity, using the free radical DPPH, the extract demonstrated antioxidant activity (IC50% = 378,80 μg L-1). However, it has not been possible to correlate the levels of antioxidant activity at levels of phenols and flavonoids. Perhaps the presence of other substances alkaloids and tannins present in the extracts may have been responsible for the antioxidant activity found. The extract was able to inhibit the growth at concentrations of 500 and 1000 mg mL-1 except Pseudomonas aeruginosa ATCC 27853, Salmonella enterica subsp. serovar Typhi ATCC 19214, all strains had the same results in both the disk diffusion test as in CIM. That way the antioxidant and antibacterial activities of EHPG 10% may represent important therapeutic potential, particularly for animal health in organic and agroecological production systems, where the use of chemical antibiotics is vetoed. It is concluded that the hydroalcoholic extract of Punica granatum Linn. has antimicrobial activity, demonstrating potential benefit for the control of bovine mastitis.