Navegando por Autor "Negreiros, Mariana Magesto de"
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- DissertaçãoAcesso aberto (Open Access)Reação de cultivares de feijoeiro comum a isolados de Fusarium oxysporum f. sp. phaseoli(Universidade Estadual do Norte do Paraná, 2018-09-24) Negreiros, Mariana Magesto de; Torres, João Pereira; https://orcid.org/0000-0002-7306-9872; http://lattes.cnpq.br/2582950118304206; Torres, João Pereira; https://orcid.org/0000-0002-7306-9872; http://lattes.cnpq.br/2582950118304206; Canteri, Marcelo Giovanetti; https://orcid.org/0000-0002-6625-5909; http://lattes.cnpq.br/8756197722397278; Carvalho, Mayra Costa da Cruz Gallo de; http://lattes.cnpq.br/2844751503530944; http://lattes.cnpq.br/2844751503530944; Bueno, João Tavares; https://orcid.org/0000-0003-0716-1141; http://lattes.cnpq.br/2889380559858950; Sumida, Ciro Hideki; http://lattes.cnpq.br/5355869058801821Fusarium’s wilt is a disease caused by the Fusarium oxysporum f. sp. phaseoli (Fop) fungus. The most effective control method is the use of resistant cultivars, but the pathogen’s physiological variability makes the resistant cultivars development difficult by the breeding programs. The objective of this paper is to characterize the reaction of the main commercial bean cultivars of Paraná’s State (Phaseolus vulgaris L.) as to resistance to different Fusarium oxysporum f. sp. phaseoli isolates. There were obtained and characterized four monosporic isolates, two from different Paraná locations (IPR7 and APR1) and two from Minas Gerais (PMG2 and PMG5). Four other isolates obtained from symptomatic plants of Paraná were identified as F. solani. The identification of the isolates was performed by PCR (SCAR-RAPD and PCR-ITS) and morphological evaluation of macro and microconidia. Fop isolates were also evaluated for pathogenesis and aggressiveness in 15 common bean cultivars. The inoculation of the bean plants was performed by the root immersion method in suspension of conidia under greenhouse conditions, and the evaluations using a CIAT severity score scale at 7, 14, 21, 28 and 32 days after inoculation. Isolates IPR7, PMG2, APR1 and PMG5, all identified as Fop, were obtained through morphological and molecular evaluation. Of the isolates produced from the P2 plant (Arapoti/PR), four were identified as F. solani (monosporic P2.3, P2.13, P2.14 and P2.15) and the other as Fop. The PMG5, PMG2, IPR7 and IAC9453 isolates were shown as intermediates as to the pathogenesis on the differentiating cultivars and on the control ones too. The evaluated common bean commercial cultivars showed intermediary resistance and reactions as to the studied isolates.