Navegando por Autor "Oliveira, João Paulo de"

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    Efeito da pressão do circuito de pulverização na taxa de sobrevivência dos agentes biológicos
    (Universidade Estadual do Norte do Paraná, 2026) Oliveira, João Paulo de; Matsumoto, Leopoldo Sussumu; https://orcid.org/0000-0001-5102-545X; http://lattes.cnpq.br/0857955043436449; Matsumoto , Leopoldo Sussumu; https://orcid.org/0000-0001-5102-545X; http://lattes.cnpq.br/0857955043436449; Dário, Gustavo; https://orcid.org/0000-0002-4648-6881; http://lattes.cnpq.br/5682942731827088; Nicolosi, Mateus Marrafon; http://lattes.cnpq.br/5740779198324583; Gandolfo, Marco Antonio; https://orcid.org/0000-0003-2314-3752; http://lattes.cnpq.br/5560552732033631; Lopes, Valdir; https://orcid.org/0000-0002-7407-7858; http://lattes.cnpq.br/0481390085872915
    The success of biological control and agricultural inoculation depends on maintaining the viability of microorganisms during the application technology process. This study aimed to evaluate the survival of Bacillus pumilus, Azospirillum brasilense, and Isaria fumosorosea subjected to different pressure levels (300 to 700 kPa) and recirculation times (0 to 40 min) within a spraying circuit. Viability was quantified through plate counting (CFU mL⁻¹) and quantitative real-time PCR (qPCR). The results demonstrated that cellular morphology is the determining factor in resilience to hydrodynamic stress. B. pumilus showed high stability across all treatments, attributed to the mechanical protection of its endospores. In contrast, A. brasilense (Gram-negative) exhibited high sensitivity, with drastic population declines (R2=0.93) as pressure and time increased, reaching non-detection via qPCR under 700 kPa. The fungus I. fumosorosea showed intermediate resistance, remaining stable up to 500 kPa but becoming vulnerable to the immediate impact of higher pressures (R2=0.76). It is concluded that extreme pressures cause structural damage and DNA fragmentation in vegetative cells and conidia. It is recommended to calibrate the working pressure according to the biological nature of the input, prioritizing low pressures and shorter recirculation times for non-spore-forming agents.