Navegando por Autor "Soares, Gabriel Lourenzo Rezende"

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    Avaliação de resíduo de sulfato de gentamicina no leite de vacas com mastite subclínica tratadas por via intramamária
    (Universidade Estadual do Norte do Paraná, 2018-03-20) Soares, Gabriel Lourenzo Rezende; Lima, Cristina Batista de; https://orcid.org/0000-0002-6076-4286; http://lattes.cnpq.br/3072842301542197; Silva, Marcelo Alves da; http://lattes.cnpq.br/3283906522734749; Porto, Petrônio Pinheiro; https://orcid.org/0000-0002-2999-7612; http://lattes.cnpq.br/1751366795715746; Campos, Anna Carolina Leonoelli Pires de; https://orcid.org/0000-0003-0222-5732
    The objectives of this study were to investigate the presence of gentamicin residue in milk of treated and adjacent mammary quarters not treated with gentamicin; verify the influence of subclinical mastitis treatment, during the lactation, on somatic cell count, fat, total protein and lactose. Five cows with somatic cell count less than 200,000 and negative in CMT (group 01, n = 5) and ten cows with somatic cell count above 200,000 and positive in CMT (group 02: one mammary quarter score 1 on CMT, n = 5 and group 03: leastwise one mammary quarter score 2 or 3 on CMT, n = 5) were treated with two intramammary infusions of 150 mg of gentamicin sulphate with a 24 hour interval between applications. The treatment was made in the anterior right mammary quarter of the animals of group 01 and in the positive mammary quarter in CMT of the animals of groups 02 and 03. Milk samples were analyzed from the animals at 0, 36, 72, 120 and 144 hours of milk of treated quarters and 0, 12, 36, 72 and 120 hours in not treated quarters after the first infusion. Milk samples were analyzed from the animals of groups 01, 02 and 03 of the milk of treated mammary quarters and the pool of untreated quarters and performed analysis of CCS and milk solids at time 0 and 15 days after treatment. A microbiological inhibition test was performed to detect gentamicin residue with a minimum sensitivity of 50 μg/L, below the maximum allowable residue limit of 200 μg/L (Delvotest® SP NT). There was no significant difference for CCS and milk solids means between samples of time 0 and 15 days after treatment in groups 01, 02 and 03 (P ≤ 0.05, in T-test for paired samples). The gentamicin residue had a decreasing frequency of positive samples in group 01 and at the end of the analyzes, at time 144 (24 hours after the end of withdrawal period) after the first infusion, presented 40% of positive samples in milk of treated mammary quarters. In milk of untreated mammary quarters all samples were negative at all times. In group 02, for milk of treated mammary quarters, there was 80% of positive samples at time 144. In milk of untreated mammary quarters all samples were negative at all times collected. In group 03, 80% of milk of treated mammary quarters samples were positive at time 144 and in milk of untreated mammary quarters, all samples were negative, however, 60% of the samples had residue close to the detection limit in time 36. Milk from untreated mammary quarters adjacent to subclinical mammary quarter treated with gentamicin sulfate does not present residue above the maximum residue limit allowed by legislation and is considered safe for human consumption. Milk from gentamicin treated mammary quarters remained positive to Delvotest® at 40%, 80% and 80% in groups 01, 02 and 03 after the end of the withdrawal period established by the manufacturer.