Navegando por Autor "Tominaga, Bruna Kanno"

Agora exibindo 1 - 1 de 1
  • Imagem de Miniatura
    Dissertação
    Parâmetros biológicos de Spodoptera cosmioides (Walker, 1858) (Lepidoptera: Noctuidae) e potencial de nematoides entomopatogênicos para manejo na soja
    (Universidade Estadual do Norte do Paraná, 2026) Tominaga, Bruna Kanno; Alves , Viviane Sandra; https://orcid.org/0000-0003-2381-8115; http://lattes.cnpq.br/3794580975938452; Alves, Viviane Sandra; https://orcid.org/0000-0003-2381-8115; http://lattes.cnpq.br/3794580975938452; Silva, Marco Aurélio Tramontin da; https://orcid.org/0000-0002-1019-1926; http://lattes.cnpq.br/8341476195396585; Rodrigues, Diego Resende; orcid.org/0000-0002-9495-8943; http://lattes.cnpq.br/9381400899886153
    Spodoptera cosmioides (Walker, 1858) (Lepidoptera: Noctuidae) is a pest of great economic importance that affects several crops, making the adoption of control measures essential once it is detected in the field. Knowledge of its life cycle, colony optimization, and the identification of efficient natural enemies are fundamental steps for the development of management programs. Thus, this study aimed to investigate the biology of S. cosmioides under different diets and to improve evaluations of the use of entomopathogenic nematodes (EPNs) in its management under laboratory and greenhouse conditions. Experiments were conducted to evaluate the biology of S. cosmioides fed on an artificial diet and soybean leaves. In addition, the survival of S. cosmioides after EPN inoculation in pupae, the foliar consumption of larvae over time after EPN inoculation, and the survival of EPNs applied to the aerial parts of soybean plants under greenhouse conditions were assessed. Differences in the life cycle duration of S. cosmioides were observed depending on the diet, both in early instars and in the pupal and adult stages. The artificial diet resulted in a shorter life cycle and higher female fecundity. In the nematode assays, EPN inoculation reduced foliar consumption of S. cosmioides by more than 65% after 24 hours. After foliar application on soybean plants in greenhouse conditions, both isolates showed 100% infectivity, causing mortality in Galleria mellonella. However, there was a rapid decline in efficacy over time, with reduced mortality after 12 hours and no effect after 36 hours, indicating loss of viability of infective juveniles (IJs). In the soil, however, survival was higher, with an average infectivity of 54.7% and stability of the UEL08 isolate over 72 hours. The effectiveness against pupae was low (0–4%), with no differences among treatments. It is concluded that the Greene diet is a viable alternative for maintaining S. cosmioides colonies, and that EPNs have potential for use in its control, as they significantly reduced foliar consumption of infected larvae. On the other hand, foliar application without adjuvants is not viable, since a rapid loss of IJ viability and infectivity was observed, indicating the need for further studies to develop strategies to enhance IJ survival over longer periods.