Programa de Pós-Graduação em Agronomia

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Navegando Programa de Pós-Graduação em Agronomia por Autor "Alves, Luis Francisco Angeli"

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    Avaliação da produção in vivo, alternativas de meio de cultura e diferentes concentrações de inóculo para produção in vitro de Heterorhabditis amazonensis (Isolado UEL 08)
    (Universidade Estadual do Norte do Paraná, 2022) Doneze, Gabriela Souza; Alves, Viviane Sandra; https://orcid.org/0000-0003-2381-8115; http://lattes.cnpq.br/3794580975938452; Alves, Viviane Sandra; https://orcid.org/0000-0003-2381-8115; http://lattes.cnpq.br/3794580975938452; Matsumoto, Leopoldo Sussumu; https://orcid.org/0000-0001-5102-545X; http://lattes.cnpq.br/0857955043436449; Alves, Luis Francisco Angeli; https://orcid.org/0000-0002-3102-764X; http://lattes.cnpq.br/0613029292752945
    Entomopathogenic Nematodes (EPN) are obligate parasites that have an association with symbiotic bacteria and cause mortality in insects, so they are excellent agents in biological control. EPN-based products are a reality outside Brazil, but here, they are still scarce due to the difficulty of large-scale production. On the other hand, the native isolate Heterorhabditis amazonensis (UEL 08) was evaluated as a potential agent for use in the control of the avian rattlesnake, Alphitobius diaperinus (Coleoptera: Tenebrionidae). Although pathogenicity and virulence tests on the insect indicate that this is a promising isolate, there is no evidence that it can be produced on a large scale and used to control this pest. Therefore, this work aimed to perform in vivo and in vitro production tests of isolate UEL 08. For the in vivo test, Galleria mellonella larvae (Lepidoptera: Pyralidae) were used as host and the production per larva, per gram of larva and total production was evaluated. In vitro production was evaluated using solid and semi-solid medium containing different fat sources (corn, canola, cotton, soybean, coconut, pork fat and bovine milk butter). Subsequently, different inoculum sizes (1,000, 2,500, 5,000, 7,500 and 10,000 Infecting Juveniles (IJs)/fry) were evaluated. The trials were conducted in an entirely randomized design, with four replicates for each treatment. In the in vivo production assay we observed a production of 6.5 × 10⁴ IJs/larva and a total production of 6.5 × 10⁵ IJs/repetition. In in vitro production in solid medium, there was a difference between the fat sources used, with corn oil providing the highest production with 926.5 IJs/mL of medium. In semi-solid medium, corn oil also showed the best production, with 3.2 × 10⁴ IJs/mL, differing from all other fat sources evaluated. The IJs produced both in solid and semi-solid medium were virulent when applied on G. mellonella larvae, with mortality values approaching 100%. The best inoculum concentration in semi-solid medium was 1,000 IJs/well.