Navegando por Autor "Leme, Matheus Eduardo"

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    Ação In vitro do extrato do botão floral da bananeira (Musa spp.) sobre nematódeos gastrintestinais de ovinos
    (Universidade Estadual do Norte do Paraná, 2018-05-15) Leme, Matheus Eduardo; Peixoto, Erika Cosendey Toledo de Mello; https://orcid.org/0000-0002-9608-4282; http://lattes.cnpq.br/0963818563395019; Silva, Marcos Augusto Alves da; https://orcid.org/0000-0002-5570-8677; http://lattes.cnpq.br/4973981237909142; Silva, Marcos Augusto Alves da; https://orcid.org/0000-0002-5570-8677; http://lattes.cnpq.br/4973981237909142; Fertonani, Luciane Holsback Silveira; https://orcid.org/0000-0001-5630-3295; http://lattes.cnpq.br/0120344533995048; Yoshihara, Eidi; https://orcid.org/0000-0002-1798-0418; http://lattes.cnpq.br/3447906791020709
    One of the main economic losses in sheep farming is gastrointestinal nematode infections. The indiscriminate use of chemicals to control these parasites has contributed to the appearance of anthelmintic resistance in several countries including Brazil. Studies carried out with the objective of controlling the parasites and reducing the impacts of resistance have related several tannins plants with anthelmintic properties, especially Musa spp. The banana tree (Musa spp.) is widely distributed mainly in countries with a tropical climate; its cultivation focused on food purposes due to its nutritional value. The present study aimed to evaluate the anthelminthic action of the hydroalcoholic extract of the floral bud of the banana tree (HEB) to 10% in eggs and larvae of gastrointestinal nematodes of sheep, as well as its antioxidant activity. Samples of naturally infected sheep were used with a minimum egg count of 2,000 eggs per gram of feces (EPG), and egg hatchability tests (EHT) and larval migration inhibition test (LMIT) were performed. In addition, total polyphenols, condensed tannins, flavonoids, and antioxidant activity of the extract were determined. Five concentrations of HEB (20; 40; 80; 160 and 320 mg mL-1), negative control with distilled water (NC) and positive control with Albendazole Sulfide (PC) were tested in quadruplicate. In relation to EHT, HEB inhibited 82.57% in the concentration of 160 mg mL-1, reaching 100% in the concentration of 320 mg mL-1. In relation to LMIT, HEB inhibited 90.30% in the concentration 80 mg mL-1, 94.80% in the concentration of 160 mg mL-1, reaching 100% in the concentration of 320 mg mL-1. The HEB (10 mg mL-1) presented 0.38 mg EAG g-1 of total polyphenols, 372.70 mg EAT g-1 of tannins, 0.42 mg RE g-1 of flavonoids, and antioxidant activity of 43.03% with IC50 corresponding to 0.2765 mg mL-1. The activity of the extract could be related to the phenolic compounds present in the extracts, specifically condensed tannins. Thus, it was possible to verify the anthelmintic and antioxidant activity of the HEB, demonstrating its potential in the control of verminosis in sheep. In addition, these results are of great importance for organic, agroecological and biodynamic production systems, whose use of synthetic parasiticides is not allowed.