Navegando por Autor "Martins, Livian Soares"

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    Dissertação
    Biocontrole de fitopatógenos: uma perspectiva integrada de produção científica e estudos experimentais
    (Universidade Estadual do Norte do Paraná, 2026) Martins, Livian Soares; Marques, Luis Eduardo Azevedo Lescano; https://orcid.org/0000-0003-0874-9211; http://lattes.cnpq.br/0477576296995064; Carvalho, Mayra Costa da Cruz Gallo de; https://orcid.org/0000-0002-1559-3828; http://lattes.cnpq.br/2844751503530944; Carvalho, Mayra Costa da Cruz Gallo de; https://orcid.org/0000-0002-1559-3828; http://lattes.cnpq.br/2844751503530944; Marques, Luis Eduardo Azevedo Lescano; https://orcid.org/0000-0003-0874-9211; http://lattes.cnpq.br/0477576296995064; Sichieri, Rafaella Cardoso; http://lattes.cnpq.br/7030413218339310; Reis, Guilherme Fonseca; http://lattes.cnpq.br/5675852978278805
    The biological control has been widely studied as a sustainable strategy for the management of plant diseases caused by phytopathogenic fungi. This work aimed to evaluate the potential of Talaromyces stollii as a biocontrol agent of the soil phytopathogenic fungi Sclerotinia sclerotiorum and Macrophomina phaseolina and to analyze the evolution of scientific production on the molecular biology of biological control of plant diseases through a scientometric study. For the laboratory assays, T. stollii strain BCA1.1 was cultivated in dual culture on PDA with S. sclerotiorum and M. phaseolina. The action of volatile compounds and metabolites present in the supernatant obtained from the antagonist fungus culture on the growth of the phytopathogens was evaluated. For the scientometric analysis of publications on molecular mechanisms of antagonism between biocontrol agents and phytopathogens, data were collected from the Web of Science and Scopus databases between 1990 and 2024, analyzing indicators of scientific production growth, main thematic areas, authors, institutions, collaboration networks, and research trends. The experimental results showed that T. stollii inhibited both phytopathogens only in direct confrontation in dual culture. Macroscopic analysis of the plate revealed that the invasion of S. sclerotiorum initially occurred beneath and then above the mycelium, while in M. phaseolina the antagonist established itself over the mycelium through sporulation. Observations by optical light microscopy, using the adhesive tape method, allowed visualization of the typical hyphal coiling of mycoparasitism and the sporulation of the antagonist over the mycelium. Volatile compounds and non-volatile metabolites of T. stollii did not inhibit fungal growth. The results suggest that inhibition depends on direct contact, indicating that T. stollii may have potential in integrated management and in reducing chemical pesticides. The scientometric analysis showed that studies on molecular mechanisms of biocontrol began in 1990 and grew significantly from 2007 onwards, with an average annual rate of 9.99%. There was a shift from the study of classical agents, such as Trichoderma and Bacillus subtilis, to modern approaches involving genomics, transcriptomics, metabolomics, enzyme production, secondary metabolites, and complex ecological interactions. Biological control has evolved from traditional practices to a science guided by “omics,” highlighting genomic prospecting and rational formulation of biological agents. China, Brazil, and India stand out, with international collaboration networks and greater participation of the global south. Future perspectives include multifunctional agents, genetic engineering, optimization of antifungal metabolites, and integration with sustainable management. The integration between laboratory results and scientometric analysis reinforces the potential of T. stollii and indicates that advances in biotechnology and molecular biology may expand its practical application in the control of agricultural diseases.