Programa de Pós-Graduação em Agronomia
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Navegando Programa de Pós-Graduação em Agronomia por Assunto "12. Consumo e produção sustentáveis"
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- DissertaçãoAtividade nematicida de extratos de Brugmansia suaveolens sobre Meloidogyne incognita(Universidade Estadual do Norte do Paraná, 2026) Venâncio, Stéphany de Campos; Alves, Viviane Sandra; https://orcid.org/0000-0003-2381-8115; http://lattes.cnpq.br/3794580975938452; Alves, Viviane Sandra; https://orcid.org/0000-0003-2381-8115; http://lattes.cnpq.br/3794580975938452; Poletto, Rodrigo de Souza; https://orcid.org/0000-0002-7034-7865; http://lattes.cnpq.br/8774064317309922; Silva, Santino Aleandro da; https://orcid.org/0000-0002-1019-1926; http://lattes.cnpq.br/8341476195396585Meloidogyne incognita stands out as one of the most aggressive and widely distributed species in tropical regions, causing severe damage to plant root systems. The need for alternatives to synthetic nematicides drives research into plant-based compounds with biological potential and lower environmental impact. In this context, the present study aimed to evaluate the nematicidal activity of aqueous and hydroalcoholic extracts from leaves and flowers of Brugmansia suaveolens (Solanaceae) on second-stage juveniles (J2) of M. incognita. The experiments were conducted under controlled laboratory conditions, using J2 obtained from infected tomato roots, processed with sodium hypochlorite solution and filtered through 250 and 500 mesh sieves. The suspensions containing J2 were exposed to two types of extracts (aqueous and hydroalcoholic) at different concentrations, and a negative control was performed with distilled water. The mortality of the juveniles was evaluated after 24 hours of exposure, and the data obtained were submitted to analysis of variance and Tukey's test (p<0.05). The aqueous extracts caused higher mortality of M. incognita J2, with the flower extract causing up to 98% mortality in all concentrations evaluated and showing no significant difference. The aqueous leaf extract, on the other hand, showed dose-dependent mortality, with values varying according to the dose tested. For the hydroalcoholic extracts, mortality was observed in the extracts obtained from flowers and leaves, with values ranging from 46 to 59% mortality. Analysis by gas chromatography coupled with mass spectrometry (GC-MS) for the detection and identification of volatile chemical constituents present in B. suaveolens extracts confirmed significant differences between the extracts, both in terms of the extraction method and the part of the plant used.
- DissertaçãoBiocontrole de fitopatógenos: uma perspectiva integrada de produção científica e estudos experimentais(Universidade Estadual do Norte do Paraná, 2026) Martins, Livian Soares; Marques, Luis Eduardo Azevedo Lescano; https://orcid.org/0000-0003-0874-9211; http://lattes.cnpq.br/0477576296995064; Carvalho, Mayra Costa da Cruz Gallo de; https://orcid.org/0000-0002-1559-3828; http://lattes.cnpq.br/2844751503530944; Carvalho, Mayra Costa da Cruz Gallo de; https://orcid.org/0000-0002-1559-3828; http://lattes.cnpq.br/2844751503530944; Marques, Luis Eduardo Azevedo Lescano; https://orcid.org/0000-0003-0874-9211; http://lattes.cnpq.br/0477576296995064; Sichieri, Rafaella Cardoso; http://lattes.cnpq.br/7030413218339310; Reis, Guilherme Fonseca; http://lattes.cnpq.br/5675852978278805The biological control has been widely studied as a sustainable strategy for the management of plant diseases caused by phytopathogenic fungi. This work aimed to evaluate the potential of Talaromyces stollii as a biocontrol agent of the soil phytopathogenic fungi Sclerotinia sclerotiorum and Macrophomina phaseolina and to analyze the evolution of scientific production on the molecular biology of biological control of plant diseases through a scientometric study. For the laboratory assays, T. stollii strain BCA1.1 was cultivated in dual culture on PDA with S. sclerotiorum and M. phaseolina. The action of volatile compounds and metabolites present in the supernatant obtained from the antagonist fungus culture on the growth of the phytopathogens was evaluated. For the scientometric analysis of publications on molecular mechanisms of antagonism between biocontrol agents and phytopathogens, data were collected from the Web of Science and Scopus databases between 1990 and 2024, analyzing indicators of scientific production growth, main thematic areas, authors, institutions, collaboration networks, and research trends. The experimental results showed that T. stollii inhibited both phytopathogens only in direct confrontation in dual culture. Macroscopic analysis of the plate revealed that the invasion of S. sclerotiorum initially occurred beneath and then above the mycelium, while in M. phaseolina the antagonist established itself over the mycelium through sporulation. Observations by optical light microscopy, using the adhesive tape method, allowed visualization of the typical hyphal coiling of mycoparasitism and the sporulation of the antagonist over the mycelium. Volatile compounds and non-volatile metabolites of T. stollii did not inhibit fungal growth. The results suggest that inhibition depends on direct contact, indicating that T. stollii may have potential in integrated management and in reducing chemical pesticides. The scientometric analysis showed that studies on molecular mechanisms of biocontrol began in 1990 and grew significantly from 2007 onwards, with an average annual rate of 9.99%. There was a shift from the study of classical agents, such as Trichoderma and Bacillus subtilis, to modern approaches involving genomics, transcriptomics, metabolomics, enzyme production, secondary metabolites, and complex ecological interactions. Biological control has evolved from traditional practices to a science guided by “omics,” highlighting genomic prospecting and rational formulation of biological agents. China, Brazil, and India stand out, with international collaboration networks and greater participation of the global south. Future perspectives include multifunctional agents, genetic engineering, optimization of antifungal metabolites, and integration with sustainable management. The integration between laboratory results and scientometric analysis reinforces the potential of T. stollii and indicates that advances in biotechnology and molecular biology may expand its practical application in the control of agricultural diseases.
- DissertaçãoEfeitos da aplicação de microrganismo eficiente sobre crescimento, produção e qualidade de frutos de Solanum Lycopersicum L.(Universidade Estadual do Norte do Paraná, 2026) Silva, Leandro de Oliveira; Kölln, Oriel Tiago; https://orcid.org/0000-0002-8507-9808; http://lattes.cnpq.br/8041626078120100; Porto, Petrônio Pinheiro; https://orcid.org/0000-0002-2999-7612; http://lattes.cnpq.br/1751366795715746; Porto, Petrônio Pinheiro; https://orcid.org/0000-0002-2999-7612; http://lattes.cnpq.br/1751366795715746; Pinto, Fabiana Gisele da Silva; https://orcid.org/0000-0002-0486-8486; http://lattes.cnpq.br/9361463429150328; Matsumoto, Leopoldo Sussumu; https://orcid.org/0000-0001-5102-545X; http://lattes.cnpq.br/0857955043436449Contemporary agriculture faces increasing challenges related to sustainability, food security, and mitigation of environmental impacts. In this context, the tomato plant (Solanum lycopersicum L.), a vegetable crop of great economic and social relevance, serves as an ideal model for studies on plant-microorganism interactions. The objective of this dissertation was to evaluate the effects of applying effective microorganisms (EMs), particularly in the form of liquid bokashi/Tomita Microorganisms Technology (TMT), on the growth, yield, and fruit quality of tomato plants, also considering their interaction with arbuscular mycorrhizal fungi (AMF). The first article presents a systematic review, conducted according to the PRISMA protocol, which identified 2,450 records and included 31 studies. These studies demonstrate the benefits of the co-inoculation of AMF and EMs, such as increased phosphorus uptake, enhanced biomass accumulation, tolerance to abiotic stresses, suppression of pathogens, and improvements in fruit quality. The second article describes an experiment conducted under controlled conditions, using a completely randomized design (CRD) with seven treatments, in addition to a control, to evaluate different rates and application frequencies of the product associated with NPK 10-10-10 mineral fertilization. Treatments T1 (50 L ha⁻¹), T2 (100 L ha⁻¹), T3 (200 L ha⁻¹), and T7 (400 L ha⁻¹) received a single application, while treatments T4 (50 L ha⁻¹), T5 (100 L ha⁻¹), and T6 (200 L ha⁻¹) received two applications at the same rate. The control received no application of the product. The evaluated variables included vegetative growth parameters (plant height, leaf number, leaf area), fresh and dry biomass accumulation, yield (fruit number and weight), and quality parameters (soluble solids content, firmness, and color). The results demonstrated that split application of TMT promoted a significant increase in plant height (up to 22% compared to the control), leaf area (average increase of 18%), dry biomass accumulation (25% increase), and yield (20% increase in both fruit number and weight). In contrast, single application concentrated effects on vegetative growth, without significant gains in reproductive output. It is concluded that the use of effective microorganisms, associated with mycorrhizal symbiosis, represents a promising strategy to strengthen tomato cultivation in sustainable agricultural systems, reducing the need for synthetic fertilizers and pesticides while reconciling productivity, quality, and environmental conservation in line with the principles of regenerative agriculture.
- DissertaçãoInfluência do condicionador de solos nos atributos químicos, microbiológico e produtividade do milho segunda safra(Universidade Estadual do Norte do Paraná, 2026) Costa, Eduardo Almeida da; Silva, Marcos Augusto Alves da; https://orcid.org/0000-0002-5570-8677; http://lattes.cnpq.br/4973981237909142; Nicolosi, Mateus Marrafon; http://lattes.cnpq.br/5740779198324583; Kölln, Oriel Tiago; https://orcid.org/0000-0002-8507-9808; http://lattes.cnpq.br/8041626078120100; Osipe, Jethro Barros; https://orcid.org/0000-0001-6453-3090; http://lattes.cnpq.br/9692340257207288; Matsumoto, Leopoldo Sussumu; https://orcid.org/0000-0001-5102-545X; http://lattes.cnpq.br/0857955043436449Soil conditioners play a fundamental role in modern agriculture, as they contribute to improved plant development and promote enhancements in soil microbiota, favoring a more balanced and active rhizosphere. In this context, the present study aimed to evaluate the efficiency of peat- and humic acid-based soil conditioners on soil microbiology and chemistry, as well as on the phytochemical parameters of corn (Zea mays L.). The experiment was conducted in a randomized complete block design (RCBD) with four replications. The treatments were as follows: T1 – Control; T2 – Methylobacterium symbioticum; T3 – 100 kg ha⁻¹ of granulated conditioner + M. symbioticum; T4 – 200 kg ha⁻¹ of granulated conditioner + M. symbioticum; T5 – 300 kg ha⁻¹ of granulated conditioner + M. symbioticum; T6 – 100 kg ha⁻¹ of powdered conditioner + M. symbioticum; T7 – 200 kg ha⁻¹ of powdered conditioner + M. symbioticum; T8 – 300 kg ha⁻¹ of powdered conditioner + M. symbioticum; T9 – 100 kg ha⁻¹ of powdered conditioner enriched with Trichoderma asperellum + M. symbioticum; T10 – 200 kg ha⁻¹ of powdered conditioner enriched with T. asperellum + M. symbioticum; and T11 – 300 kg ha⁻¹ of powdered conditioner enriched with T. asperellum + M. symbioticum. In the control treatment, 300 kg ha⁻¹ of urea was applied, while in the treatments containing M. symbioticum, there was a 30% reduction in the urea dose. The following parameters were evaluated: plant height, shoot dry mass (SDM), root dry mass (RDM), microbiological attributes, soil chemical analysis, thousand-grain weight (TGW), and final yield (kg ha⁻¹). It was observed that treatment T11, with 300 kg ha⁻¹ of powdered conditioner + Methylobacterium symbioticum, showed an increase of 1,190.4 kg ha⁻¹ compared to the control. Furthermore, all treatments with soil conditioners promoted improvements in soil microbiological attributes. Therefore, it can be concluded that soil conditioners play an important role, and the best treatment among those evaluated was the one containing powdered soil conditioner enriched with Trichoderma asperellum + Methylobacterium symbioticum.
- DissertaçãoTerbutilazina na entressafra: período de segurança pré-semeadura e seletividade para soja em solos de textura contrastantes(Universidade Estadual do Norte do Paraná, 2026) Osipi, Carlos Botelho Pereira; Osipe, Jethro Barros; https://orcid.org/0000-0001-6453-3090; http://lattes.cnpq.br/9692340257207288; Kölln, Oriel Tiago; https://orcid.org/0000-0002-8507-9808; http://lattes.cnpq.br/8041626078120100; Osipe, Jethro Barros; https://orcid.org/0000-0001-6453-3090; http://lattes.cnpq.br/9692340257207288; Gustavo, Dário; https://orcid.org/0000-0002-4648-6881; http://lattes.cnpq.br/5682942731827088; Dias, Roque de Carvalho; https://orcid.org/0000-0001-5433-5373; http://lattes.cnpq.br/5490315867189084Chemical weed control during the off-season allows interventions when weeds are still at earlier growth stages and broadens the range of available herbicide modes of action, which supports herbicide rotation. However, this practice also raises concerns about residual activity on the succeeding crop and the potential risk of crop injury. Terbuthylazine has emerged as a potential alternative to atrazine. Understanding its behavior in soils with contrasting textures, as well as its potential to injure currently used soybean cultivars, is essential for establishing safe recommendations. This study aimed to: (i) determine the minimum interval required between terbuthylazine application and soybean sowing in sandy- and clay-textured soils; and (ii) identify differences in soybean cultivar sensitivity to terbuthylazine. To address objective (i), two field experiments were conducted in Assis, São Paulo State, Brazil (sandy Oxisol, 15% clay) and Bandeirantes, Paraná State, Brazil (clayey Rhodic Eutrudox, 60% clay), using terbuthylazine rates of 0.6 and 0.8 kg a.i. ha⁻¹ applied at 48, 36, 24, 12, and 0 days before soybean sowing (cultivar NK 6630). For objective (ii), the sensitivity of five soybean cultivars was evaluated under greenhouse conditions based on crop injury, chlorophyll content (SPAD index), fresh mass, and dry mass. In the sandy soil, the minimum safe interval was 32 days for both herbicide rates. In the clayey soil, no effect on yield was observed, regardless of rate or application timing. Genotypic variability in cultivar response was observed: NK 6630 was the most sensitive material, showing greater crop injury and lower SPAD values, whereas Monsoy 6601 was the most tolerant, with lower levels of crop injury.